Wednesday Seminar: click beetles

Molecular identification and genetic diversity of economically important click beetles
by Dr Carly Benefer (Post-Doctoral Research Fellow, Plymouth University)

Venue: LT PLYMOUTH, PSQ
Date: Wednesday 10 October 2012
Time: 1-2pm

The larvae of click beetles (wireworms) are important crop pests worldwide. There are many damage-causing species but identification is difficult, ambiguous and time-consuming. For sustainable pest management it is important to know which species are involved in damage so that they can be targeted, and for that reason information on the relationships, population genetics and phylogeography of such species is useful

Recently progress has been made in identifying European species using molecular methods, and subsequent studies have found that even closely related species can have distinct life histories. However, in Canada there are many species of economic importance that are difficult to identify (and many are undergoing taxonomic revision) but for which such molecular techniques are lacking. In addition, invasive species from Europe and re-emergence of pest species are causing widespread damage in some locations.

In this talk I’ll describe a study which aimed to assess the genetic variation and phylogenetic relationships of wireworms collected in agricultural land across Canada using 16S rRNA sequence data.

Haplotype map based on 16S rRNA sequences of Hypnoidus
bicolor wireworms in sampling locations across Canada

Adult click beetle

Wireworm

Exciting opportunities for volunteering alongside your degree…

Last weekend students from Biological Sciences and Coastal Ecology courses volunteered with OPAL and helped run a two-day Bioblitz at Pentillie Estate near Saltash. They helped us to engage over 300 people with wildlife and their local environment, and helped to discover Greater and Lesser Horseshoe bats, not previously known to be at the site, as well as Violet Ground Beetles, Lesser Stag Beetles and a plethora of plant, bird and invertebrate species not recorded there before.

If you would like to help with similar events then OPAL has plenty of volunteering opportunities coming up that anyone can get involved in. You can improve your identification skills and gain essential experience of field surveys and public engagement with science. It is also a great way to get to know the local area, meet wildlife professionals and get a sense of the sort of career you might enjoy.

Time commitment is flexible. Listings of current opportunities can be found here or you can email me for more info: alison.smith@plymouth.ac.uk.  

Hummingbird foraging behaviour and plant reproductive output

A shining sunbeam (Aglaeactis cupripennis) feeding on
a Puya hamata inflorescence. (Photo: Paul Ramsay)

A new study on plant-pollinator interactions by Paola García-Meneses and Paul Ramsay is about to be published in Basic and Applied Ecology. It is the first to show that the relationship of a plant to its neighbours within patches affects pollinator visits and reproductive output of the plants.

It is well known that insect and bird pollinators are attracted to patches of flowers where rewards are greater. In fact, some birds set up territories around the plants and chase away others. Typically, the idea goes, plants in big patches get more visits than plants in smaller ones, and individual, isolated plants would be expected to get the fewest visits. This sometimes leads to better reproductive output of the plants in larger patches. But this may not always be the case...

Induction week

On the boat to Mt Edgcumbe

The way that University weeks work here at Plymouth for our department means that, just a day after saying goodbye to one cohort at graduation, the next cohort of first years arrive to start their university journey.

Induction week is packed full of things to do for our new students and is a mix of serious activities and admin as well as some more fun activities (quizzes, days out, drinks receptions....).

Today marked the annual outing to Mt Edgcumbe on the Cornish side of the Tamar. Many thanks to Mount Edgcumbe House and Country Park for giving us permission to go there and do our surveys.

Graduation

Today was graduation day for the class of 2012. Our graduation ceremonies are held on Plymouth Hoe, overlooking Plymouth Sound and we were lucky again with the weather. The photos tell their own story really:

Departmental Promotional Video

Some Azores Photos

I had a few nice photos left over that didn't really fit anywhere else. If any other contributors (or students) also have photos then either add them or send them to me:

Gryllus bimaculatus

Azores Day 7 - the last day!

As Saturday began, most of us were still celebrating the end of the course in one of the big squares in Ponta Delgada where they were serving caipirinhas! However, the bus to the airport was at 6 AM so celebration time was limited......

A few struggled to get out of bed with so little sleep. To be fair, setting the alarm, but sleeping with headphones on, was never going to turn out well.

Leaving Ponta Delgada

The birthday boy may have over indulged slightly....

Finally, we have to give many thanks to our colleagues in the Azores, Nucha, Carmo, Andrea and Paola for all of their hard work - we really appreciate what you do for us and we couldn't do it without your help. Also, the staff would like to pass on our great appreciation to all of the students for working hard, and making our job easier and the field course really enjoyable.

Azores Day 6

Our last full day on the island was mostly spent at the University of the Azores. There were two lab sessions and time to prepare a scientific paper on the restored forest survey we did earlier in the week.

One lab session looked at the bacterial plates from the water samples collected at the beginning of the week. Disconcertingly, there was obvious contamination with faecal bacteria of a pool we all swam in on Monday! We also extracted chlorophyll from the water samples as a way of quantifying the presence of Cyanobacteria.

Another lab session looked at stomatal density of a range of plant species from the impressions taken yesterday.

There were two assessments today, as well. The first was the "Ultimate Test" on everything we had done on the course. And by 10 pm, each of the ten groups was to hand in their scientific paper on the forest restoration work we did earlier this week. Some met this deadline calmly, with plenty of time to spare (in some cases more than eight hours), but a few groups left things a bit late. One group in particular was in real panic a minute before the deadline. You know who you are...

The "Ultimate Test"

 A few photos from outside the labs today:

Island Canary (endemic to Macronesia)

The view west along the south coast from Lagoa towards Ponta Delgada

Once the assessments were over, it was time to celebrate the end of the field course, and Jason's birthday. After a gentle start by the waterfront, the festivities moved on to a square where locals and tourists mingle at weekends, and sample the occasional caipirinha. As midnight struck, most of us were still there, and this blog entry runs seamlessly into the next...

Azores Day 5

Back in the field today for the last time (lab day tomorrow) at two sites in the centre of the island, Lagoa do Fogo and Caldeira Velha. As usual, the weather was not great at Lagoa do Fogo....

The bus overheated on the steep climb

Lagoa do Fogo

Working on the hillside

Today was all about looking at whether there are any differences between the endemic and invasive plants that give the invasives some sort of advantage. Field measurements included chlorophyll fluorescence using a PEA (photosynthetic efficiency analysis) meter, leaf thickness and we also took  leaf impressions (using a dental product) to look at stomatal density in the lab tomorrow.

Working on the steep crater rim
above the lake

Taking "dental" impressions of the undersides
of the leaves of one of the endemic
trees, Laurus azorica.

Noting leaf dimensions.

Hedychium gardneranium - an invasive plant

A masterclass in keeping your notebook dry.

Innovative use of PEA meter clips.

Leaf impressions

 Further down the volcano, at Caldeira Velha, a different selection of plants provided further data while the "thermal" pool allowed a surprisingly cold swim!

Caldeira Velha

Some flexed their muscles...

...others felt compelled to bring
inflatable elephants.

Some even felt compelled to dance......

Every day we are seeing what seem like familiar species but most of them are endemic species or subspecies and look a little different:

"Atlantis" (Azorean) Herring Gull

Sao Miguel Grayling (Hipparchia miguelensis)

On the way back to the university for food, several of the staff made a detour to the coast for a fruitless search for an endemic water beetle in the pools in the splash zone. Maybe next year...

Azores Day 4

As well as work in the field, we do some work in the lab on this field course and today was the first of our lab days. This is only possible thanks to the kind hospitality offered to us by the biology department here at the Univesidade dos Açores. 

The two lab activities that we do are based around the work on the first day. Firstly, we look at the potential for pesticide resistance due to over use of deltamethrin in Pseudaletia caterpillars using an esterase protocol.

John Moody demonstrating our portable spectrophotometers, and one of the groups following the protocol.

Secondly we look for both Cyanobacteria and E. coli/Enterococcus in the lake and bathing water we collected on Sunday/Monday. 

Checking the samples under the microscope and identifying key organisms.

 

The University campus offers a nice setting for lunch!

John "Comedy Grandad" Moody scientifically testing the
consistency of upside-down rice pudding!